Pathway of Chitin Synthesis and Cellular Localization of Chitin Synthetase in Mucor rouxii
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چکیده
منابع مشابه
Pathway of chitin synthesis and cellular localization of chitin synthetase in Mocor rouxii.
Cell-free extracts of ikfucor rouxii contain enzymes which synthesize uridine diphosphate &lcetyl-D-glucosamine from IV-acetyl-n-glucosamine. Procedures are described to prepare radiolabeled UDP-GlcNAc using cell-free extracts or living cells of this fungus. This sugar nucleotide serves as the precursor for chitin synthesis. Free N-acetyl-n-glucosamine is a strong activator of chitin synthesis....
متن کاملProperties of a particulate chitin synthetase from Mucor rouxii.
The properties and behavior of a “microsomal” (100,000 X g particles) chitin synthetase of Mucor rouxii were investigated. The enzyme utilizes uridine diphosphate N-acetylD-glucosamine (UDP-GlcNAc) as glycosyl donor and is strongly and specifically activated by free N-acetyl-o-glucosamine (GlcNAc). A variety of GlcNAc analogues were tested as activators but were found ineffective. A small propo...
متن کاملProperties of chitin synthetase in isolated chitosomes from yeast cells of Mucor rouxii.
Chitin synthetase was isolated and purified 120-fold from the supernatant fraction (54,500 X g) of broken yeast cells of Mucor rouxii. The purified preparations consisted mainly of chitin synthetase particles (chitosomes) with an average size larger than 7 X 10(6) daltons (by gel filtration) and an average sedimentation coefficient of 105 S. The samples also contained other enzyme complexes (fa...
متن کاملProperties of Chitin Synthetase in Isolated Chitosomes from Yeast Cells of Mucor rouxii*
Chitin synthetase was isolated and purified 120-fold from the supernatant fraction (54,500 x g) of broken yeast cells of Mucor rouxii. The purified preparations consisted mainly of chitin synthetase particles (chitosomes) with an average size larger than 7 x 10” daltons (by gel filtration) and an average sedimentation coefficient of 105 S. The samples also contained other enzyme complexes (fatt...
متن کاملBioconversion of chitin to chitosan: purification and characterization of chitin deacetylase from Mucor rouxii.
Chitin deacetylase, the enzyme that catalyzes the hydrolysis of acetamido groups of N-acetylglucosamine in chitin, has been purified to homogeneity from mycelial extracts of the fungus Mucor rouxii and further characterized. The enzyme exhibits a low pI (approximately 3). Its apparent molecular mass was determined to be approximately 75 kDa by sodium dodecyl sulfate/polyacrylamide gel electroph...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62132-5